A novel trypsin inhibitor from the hemolymph of the horseshoe crab Limulus polyphemus
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چکیده
منابع مشابه
A novel trypsin inhibitor from the hemolymph of the horseshoe crab Limulus polyphemus.
Trypsin inhibitory activity from the hemolymph of Limulus polyphemus was found to co-purify with coagulogen (the clottable protein in blood coagulation) after acidification, ammonium sulfate precipitation, and gel filtration. Limulus trypsin inhibitor (LTI) was separated from coagulogen by ion-exchange chromatography on carboxymethyl-Sephadex. LTI is an inhibitor of trypsin (Ki = 3.3 nM) on bot...
متن کاملA homologue of alpha 2-macroglobulin purified from the hemolymph of the horseshoe crab Limulus polyphemus.
A high molecular weight protease inhibitor has been purified from the cell-free plasma of the horseshoe crab Limulus polyphemus using high speed centrifugation, polyethylene glycol precipitation, and gel filtration. The inhibitor is sensitive to mild acidification, methylamine treatment, and inhibits the proteolytic activity of a variety of endopeptidases. The molecule does not inhibit trypsin-...
متن کاملBlood Collection from the American Horseshoe Crab, Limulus Polyphemus
The horseshoe crab has the best-characterized immune system of any long-lived invertebrate. The study of immunity in horseshoe crabs has been facilitated by the ease in collecting large volumes of blood and from the simplicity of the blood. Horseshoe crabs show only a single cell type in the general circulation, the granular amebocyte. The plasma has the salt content of sea water and only three...
متن کاملCardiac Muscle of the Horseshoe Crab, Limulus Polyphemus
The fine structure of the cardiac muscle of the horseshoe crab, Limulus polyphemus, has been studied with respect to the organization of its contractile material, and the structure of its organelles and the cell junctions. Longitudinal sections show long sarcomeres (5.37 micro at L(max)), wide A bands (2.7 micro), irregular Z lines, no M line, and no apparent H zone. Transverse sections through...
متن کاملDevelopmental ecology of the American horseshoe crab Limulus polyphemus
During spawning events, horseshoe crab eggs are released from the female’s oviducts, and fertilized by one or more males. Eggs are shaped by the female into discrete clutches deposited in nests at depths of 10−20 cm on intertidal estuarine beaches. Distinguishing between fresh eggs and the early developmental stages is obfuscated by the large amount of dense, opaque yolk. The first unambiguous ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1991
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)52218-3